Fig. 4

Significant changes in brain (top) and plasma (bottom) levels of neurochemicals were detected following gavage of a complex Cannabis extract (n = 3,4). Mass spectrometry was used to quantitate neurochemicals in brains and plasma of mice at study end. Mice received either vehicle control or crude Cannabis extract by gavage at the following doses of CBD and THC, respectively: 10 and 0.240 mg/kg/day (low), 20 and 0.480 mg/kg/day (medium), or 50 and 1.2 mg/kg/day (high). See also Table 1. Trp = Tryptophan, Phe = Phenylalanine, NAM = Niacinamide, N-MetNic = N-methylnicotinamide. Significance thresholds are shown following ANOVA and uncorrected Fisher’s LSD post hoc analysis (*p < 0.05, **p < 0.01, ***p < 0.001)