Fig. 2

Significant changes (ANOVA p < 0.0001) in plasma levels of Δ9-THC and 11-OH-THC were determined following i.p. injection of THC/CBD (n = 3). Phytocannabinoids were measured in plasma using mass spectrometry at study end, approximately 48 h following the last dose. THC was dosed at 10 mg/kg/day for 14 days while CBD was given at 10 (1:1, Low), 50 (5:1, Medium), and 100 (10:1, High) mg/kg/day. As described in the methods, levels of phytocannabinoids for several mice were below the method limit of quantitation (LOQ) and, therefore, results are expressed in peak areas. Results for plasma phytocannabinoids are shown on the top graphs while results for brain phytocannabinoids are shown on the bottom graph. Significance thresholds are shown following Tukey post hoc analysis (*p < 0.05, **p < 0.01, *** p < 0.001)