Fig. 3

Awake time (left), and NREM (center) and REM (right) sleep as a percentage of total recording time quantified from 6 h recordings of conscious chronically-instrumented rat experiments.. Vehicle (25% DMSO in PBS) or dronabinol (10 mg/kg) was injected IP in combination with vehicle or CB₁ receptor (AM 251, 5 mg/kg) or CB₂ receptor (AM 630, 5 mg/kg) antagonist, or both. Dronabinol and a combination of dronabinol and CB₂ antagonist significantly reduced REM sleep. Data (mean ± SEM) were analyzed using linear mixed model analysis with repeated/fixed measures (CB agonist and CB antagonist) followed by post hoc multiple comparison tests with Sidak’s correction if there were significant main effects or a significant interaction of main effects. *p < 0.05