Table 1 The nucleotide sequences of the primers used
Target gene | Forward 5’-3’ | Reverse 5′-3′ |
|---|---|---|
GAPDH | AAGGGCTCATGACCACAGTC | GGATGCAGGGATGATGTTCT |
COX 2 | GATGACGAGCGACTGTTCCA | TGGTAACCGCTCAGGTGTTG |
IL 6 | GACTTCCAGCCAGTTGCCTT | GCAGTGGCTGTCAACAACAT |
CRP | GCAGTAGGTGGGCCTGAAAT | CCCGTCAAGCCAAAGCTCTA |
P21 | GAGAACTGGGGAGGGCTTTC | TCCTGAGCCTGTTTCGTGTC |
VEGR | CGGGCCTCTGAAACCATGAA | GCTTTCTGCTCCCCTTCTGT |
AKT 2 | ACAGACTGTGCCCTGTCCAC | CGTGGCCTCCAGGTCTTGAT |
MMP-2 | AGAGGATACCCCAAGCCACT | AAAGGCAGCGTCTACTTGCT |
P27 | GACTCACTCGCGGCTCC | GGCTCCCGTTAGACACTCTC |
AFP | CACCATCGAGCTCGGCTATT | TCCCAAAAACTCGCTTGGGT |
P53 | CCCCTGAAGACTGGATAACTGT | TCTCCTGACTCAGAGGGAGC |
BAX | AGGACGCATCCACCAAGAAG | CAGTTGAAGTTGCCGTCTGC |
XIAP | TGCATAATGAGGACTGGGGG | GCCCCGATCAGGAAAAACAC |
BAD | CTTGAGGAAGTCCGATCCCG | GCTCACTCGGCTCAAACTCT |