Table 1 HPLC and GC-MS settings for quantification of cannabidiol (CBD) concentrations

HPLC column temperature

30°C

HPLC flow rate

1.5 mL/min

HPLC analysis time

10 min

HPLC injection volume

5 µL

HPLC mobile phase conditions

Mobile phase A: aqueous ammonium formate (5mM)/formic acid (0.1%)

Mobile phase B: acetonitrile/formic acid (0.1%)

Isocratic, 25% mobile phase A

GC-MS carrier gas

Hydrogen at 1 mL/min

GC-MS inlet temperature

200°C

GC-MS injection volume:

1 µL at 50:1 split ratio

GC-MS temperature programme

60°C and held for 2 min, increasing to 300°C at 8°C/min and held for 10 min

GC-MS ion source temperature

230°C

GC-MS mass analyzer temperature

150°C

GC-MS analysis time

42 min

GC-MS scan range

40–600 m/z at 1 Hz