Fig. 3
Effect of polar fractions (PFs) of different cannabis varieties on pharyngeal pumping activity in C. elegans. The nematode C. elegans was exposed to the PFs from different cannabis varieties as well as pure cannabinoids. A Overview of pharyngeal pumping activity of 100 μg/mL PF of all cannabis varieties used in this study; B dose-response with 5, 50, and 100 μg/mL PF extracts of the two varieties MGC 1101 and MGC 1013 that showed an effect on the pharyngeal pumping activity; C: pharyngeal pumping activity in nematodes treated with pure cannabinoids containing 0.0143 μg/mL cannabidiol (CBD) (similar to the concentration in the polar fraction (PF) of MGC 1101), pure cannabinoids containing 0.0125 μg/mL CBD (similar to the concentration in the PF of MGC 1074), pure cannabinoids contain 0.0262 ug/mL Δ9-tetrahydrocannabinol-acid (THCA) and 0.0133 μg/mL Δ9-tetrahydrocannabinol (THC) (similar to the concentration in the PF of MGC 1101), and pure cannabinoids contain 0.0278 μg/mL THCA and 0.0140 μg/mL THC (similar to the concentration in the PF of MGC 1106). Mann-Whitney U tests (A) and t tests (B) were used to compare the vehicle control conditions ethanol (A) and Dimethyl sulfoxide (DMSO) (B) to the experimental conditions. Milli-Q water (MQ) is the water control in A and B. Green bars denote pharyngeal pumping activity in C. elegans exposed to polar fractions (PFs) of cannabis; brown bars denote vehicles; *** = false discovery rate (FDR)-corrected p < .001; ** = FDR-corrected p < .01; * = FDR-corrected p < .05