Fig. 9

Effect of THC or CBD treatment on gut microbiota of mice fed HFCD. A Relative abundance of phyla. B Bacteroidetes to Firmicutes ratio. Relative abundance of C Clostridia, D Ruminococcaceae, EBilophila, FMucispirillum, GSchaedleri. Male C57BL/6J mice aged 7–8 weeks were fed either normal diet (ND, n = 8), high fat + 1%(w/w) cholesterol + 0.5%(w/w) cholate diet (HFCD, n = 8), HFCD containing CBD (2.39 mg/kg) (HFCD+ CBD, n = 8), HFCD containing THC (2.5 mg/kg) (HFCD+THC, n = 8). Bacterial DNA was extracted, and values were expressed as mean ± SEM. Columns marked with different letters (a, b) are significantly different (p < 0.05) in the Tukey–Kramer test