Fig. 6

Effect of THC or CBD treatment on phosphorylation of carbohydrate and lipid metabolism-related proteins in mice fed HFCD. Male C57BL/6J mice aged 7–8 weeks were fed either normal diet (ND, n = 8), high fat + 1%(w/w) cholesterol + 0.5%(w/w) cholate diet (HF-C, n = 8), HFCD containing CBD (2.39 mg/kg) (HF-C CBD, n = 8), HFCD containing THC (2.5 mg/kg) (HF-C THC, n = 8). At the conclusion of the experiment, total protein was isolated from harvested livers and phosphorylated protein levels quantified. Values are expressed as phosphorylated/total protein ratio compared to ND ± SEM. Values marked with different letters are significantly different (p < 0.05) in the Tukey–Kramer test