Table 1 Primer sequences and optimal annealing temperatures for single PCR reactions
From: Massively parallel sequencing of Cannabis sativa chloroplast hotspots for forensic typing
Region | Forward primer | Reverse primer | Product size (bp) | Ta (°C) |
|---|---|---|---|---|
trnK-matK-trnK | ACGAGCCAAAGTTTTAACACAGG | TCGGCTTTTAAGTGCGGCTA | 2111 | 69 |
rps16 | AGAAAAGGGTGTAGACGAACG | TCGTTTCTCGGAGGCAAGAAT | 1398 | 66 |
trnS-trnG | TCTAATGATCCGGGGCGTAA | TGCATTCAAAACGACCTGC | 1668 | 66 |
ycf3 | ACGGCTCAGCAGTCAAGTTC | TTCGAAATTCATGAAAGGCCCC | 2095 | 68 |
accD-psaI | GGCTGTTCAAACAGGTACAGG | TGCCGGAAATACTAAGCCCA | 1424 | 68 |
clpP | TAAATTCCCCTGTCGGTGCC | ATGCCTATTGGTGTTCCAAAAGTA | 1984 | 66 |
rpl32-trnL | GGAAAAACCCACATACGGCG | TAACACTCGGCGCGGTTATT | 1964 | 69 |