Fig. 3

Inhalant cannabichromene (CBC) reduced ARDS-like induced inflammatory indices. Flow cytometry analysis of A whole blood and B lung tissues showed that Intranasal administration of Poly(I:C) effectuated a significant increase of pro-inflammatory cytokines including IL-6, IL-17, and IFNγ (PIC group) compared to the control group (sham). These effects were reversed with inhalant CBC (PIC + CBC), resulted in marked downregulation of all three pro-inflammatory cytokines (*p<0.03, means +/− SD). Further, the flow cytometry analysis of whole blood in the PIC group showed increased granulocytes (G) and monocytes (M) while lymphocytes were decreased compared to mice treated with inhalant CBC (PIC + CBC) supporting the notion that inhalant CBC can reverse the adverse effects of ARDS and re-establish the immune balance, resolving lymphopenia and restoring homeostasis