Table 4 Comparative Δ9-tetrahydrocannabinol (THC) extraction protocols from dried blood spots, of previous studies
Reference | Thomas et al. (2012) | Mercolini et al. (2013) | Kyriakou et al. (2016) | Protti et al. (2017) |
|---|---|---|---|---|
Paper Type | Sartorius TNF Card® | Whatman 903® | Whatman 903® | Whatman FTA™ DMPK-C Card® |
Extraction Solution | Step 1. 100µL of MeOHa + 400µL of TBMEb Step 2. 300µL of acetone | 1 mL of MeOHa | 990µL of MeOHa and 10µL of ISb | 1 mL of MeOHa |
Tube | Polypropylene | Vial | Not informed | Vial |
Procedures | Step 1. 45 min of sonication and 5 min of centrifugation (13,000 × g) Step 2. 30 min of sonication | 1 min of vortexing and 5 min of centrifugation (1400 × g) | 15 min of sonication and 5 min of centrifugation (3500 × g) | 1 min of vortexing and 5 min of centrifugation (4000 rpm) |
Solvent Evaporation | Vacuum centrifuge, 40ºC | Vacuum | Vacuum | Nitrogen Stream |
Extraction Recovery | 19% | 83% | 81% | 92% |