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Table 4 Comparative Δ9-tetrahydrocannabinol (THC) extraction protocols from dried blood spots, of previous studies

From: Study of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) extraction FROM dried oral fluid spots (DOFS) and LC–MS/MS detection

Reference Thomas et al. (2012) Mercolini et al. (2013) Kyriakou et al. (2016) Protti et al. (2017)
Paper Type Sartorius TNF
Card®
Whatman 903® Whatman 903® Whatman FTA™
DMPK-C Card®
Extraction Solution Step 1. 100µL of MeOHa + 400µL of TBMEb
Step 2. 300µL of acetone
1 mL of MeOHa 990µL of MeOHa and 10µL of ISb 1 mL of MeOHa
Tube Polypropylene Vial Not informed Vial
Procedures Step 1. 45 min of sonication and 5 min of centrifugation (13,000 × g)
Step 2. 30 min of sonication
1 min of vortexing and 5 min of centrifugation (1400 × g) 15 min of sonication and 5 min of centrifugation (3500 × g) 1 min of vortexing and 5 min of centrifugation (4000 rpm)
Solvent Evaporation Vacuum centrifuge, 40ºC Vacuum Vacuum Nitrogen
Stream
Extraction Recovery 19% 83% 81% 92%
  1. aMeOH: methanol; bTBME: tert-butyl-methyl-ether; cIS: internal standards