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Table 3 Five protocols to extract cannabis by dynamic maceration (Romano and Hazekamp 2013)

From: Processing and extraction methods of medicinal cannabis: a narrative review

Cannabis (g) 5 5 5 5 10
Solvent (mL) Naphtha (200) Petroleum ether (200) Ethanol (200) Olive oil (20) + water (70) Olive oil (100)
Extraction/filtration 1 5 g Cannabis + 100 mL solvent, agitate 20 min 5 g Cannabis + 100 mL solvent, agitate 20 min 5 g Cannabis + 100 mL solvent, agitate 20 min 5 g Cannabis + 20 mL olive oil + 50 mL water; heat at 100 °C 60 min 10 g cannabis + 100 mL olive oil; heat at 100 °C 120 min
    Cool Cool
Paper filtration Paper filtration Paper filtration Paper filtration with pressure Paper filtration with pressure
Extraction/filtration 2 100 mL solvent, agitate 20 min 100 mL solvent, agitate 20 min 100 mL solvent, agitate 20 min 20 mL of hot water  
Paper filtration Paper filtration Paper filtration Paper filtration with pressure -
Combine extracts Combine extracts Combine extracts Combine extracts  
Extract clean up None None Optional activated charcoal filtration None None
Evaporation/separation Boiling water bath with nitrogen stream Boiling water bath with nitrogen stream Boiling water bath with nitrogen stream Phase separation; freeze; decant oil phase None
Reconstitution Reconstitute residue with ethanol to 100 mL Reconstitute residue with ethanol to 100 mL Reconstitute residue with ethanol to 100 mL   Collect the oil
Extract concentration (cannabis/solvent) 5 g/100 mL 5 g/100 mL 5 g/100 mL 5 g/20 mL 10 g/100 mL
Dilution factor for analysis 20 ×  20 ×  20 ×  100 ×  40 × 
Final concentration (cannabis/solvent) 2.5 mg/mL 2.5 mg/mL 2.5 mg/mL 2.5 mg/mL 2.5 mg/mL