Fig. 2
The number of c-Fos-positive neurons in the hypothalamic nuclei in response to HU-580. A The significant increase in c-Fos expression in hypothalamus nuclei from HU-treated rats ((0.1 or 100 μg/kg; i.p.) as compared to controls (F(2, 12) = 24–738; P < 0.0001). The post hoc analysis showed significant differences among the experimental data (Scheffé’s post hoc test: control vs. HU-580 (0.1 μg/kg, P < 0.01; control vs. HU-580 (100 μg/kg), P < 0.0001; HU-580 (0.1 μg/kg) vs. HU-580 (100 μg/kg), P < 0.01). B The Pearson’s correlation coefficient analysis with a significant and positive relationship between the used doses of HU-580 (0.1 or 100 μg/kg; i.p.) and the Fos immunoreactivity (r = 0.6, P < 0.0002). Finally, the linear regression analysis showed that administrations of different doses of HU-580 predicted the enhancement in the number of Fos expression in the hypothalamic nuclei (R2 = 0.6, P < 0.0005; B)