Fig. 1

Trace fear-conditioning produced a tone-associated memory in all treatment groups of female mice. a. Time course of the average percent freezing during memory testing for vehicle-treated mice that received paired conditioning, unpaired conditioning, and non-conditioning. Shaded bars show periods during which the CS was presented. b. Percent freezing was averaged within treatment groups for each conditioning type (black - paired conditioning, dark grey - unpaired conditioning, and light grey - non-conditioned). Auditory-cued memories were validated by statistically comparing freezing levels between mice that received paired versus unpaired conditioning within each treatment group using one-tailed student’s t-tests (*p < 0.05). Comparison of freezing responses between either treatment group that received paired conditioning were not statistically significant (NS) in comparison to control. N = 12 animals per group with standard errors indicated. The timeline for the experiment is shown in the inset above