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Table 1 Mass spectrometer compound parameters for cannabinoids and internal standard (IS)

From: Regulatory sampling of industrial hemp plant samples (Cannabis sativa L.) using UPLC-MS/MS method for detection and quantification of twelve cannabinoids

Cannabinoid Mass transition (m/z) Cone voltage (V) Collision energy (V)
CBDV 287.1 > 165.1 6 24
THCV 287.1 > 165.1 2 22
CBN 311.2 > 223.1 2 20
CBC 315.2 > 193.1 44 18
CBD 315.2 > 193.1 30 18
Δ-8-THC 315.2 > 193.1 26 22
Δ-9-THC 315.2 > 193.1 4 18
CBL 315.2 > 235.2 28 16
CBG 317.2 > 109.0 26 32
Δ-9-THC-D3 (IS) 318.3 > 196.1 72 24
11-nor-9-COOH-Δ-9-THC-D9 (IS) 352.1 > 194.3 68 26
THCA 356.9 > 245.1 4 30
CBDA 357.1 > 107.0 4 34
CBGA 359.2 > 136.0 36 32
  1. All transitions (m/z) were selected and compound parameters optimized for each individual cannabinoid (Δ-9-THC-D3 Δ-9-tetrahydrocannabinol-D3, 11-nor-9-COOH-Δ-9-THC-D9 11-nor-9-carboxy-Δ-9-THC-D9, CBDV Cannabidivarin, CBG Cannabigerol, CBD Cannabidiol, THCV Tetrahydrocannabivarin, CBN Cannabinol, Δ-9-THC Δ-9-tetrahydrocannabinol, Δ-8-THC Δ-8-tetrahydrocannabinol, CBL Cannabicyclol, CBC Cannabichromene, CBDA Cannabidiolic acid, CBGA Cannabigerolic acid, and THCA Tetrahydrocannabinolic acid) using Intellistart™ feature of MassLynx® or by manual optimization, as necessary. (V voltage, m/z mass-to-charge ratio, IS internal standard)