Regulatory sampling of industrial hemp plant samples (Cannabis sativa L.) using UPLC-MS/MS method for detection and quantification of twelve cannabinoids

Table 1 Mass spectrometer compound parameters for cannabinoids and internal standard (IS)

Cannabinoid	Mass transition (*m/z*)	Cone voltage (V)	Collision energy (V)
CBDV	287.1 > 165.1	6	24
THCV	287.1 > 165.1	2	22
CBN	311.2 > 223.1	2	20
CBC	315.2 > 193.1	44	18
CBD	315.2 > 193.1	30	18
Δ-8-THC	315.2 > 193.1	26	22
Δ-9-THC	315.2 > 193.1	4	18
CBL	315.2 > 235.2	28	16
CBG	317.2 > 109.0	26	32
Δ-9-THC-D3 (IS)	318.3 > 196.1	72	24
11-nor-9-COOH-Δ-9-THC-D9 (IS)	352.1 > 194.3	68	26
THCA	356.9 > 245.1	4	30
CBDA	357.1 > 107.0	4	34
CBGA	359.2 > 136.0	36	32

All transitions (m/z) were selected and compound parameters optimized for each individual cannabinoid (Δ-9-THC-D3 Δ-9-tetrahydrocannabinol-D3, 11-nor-9-COOH-Δ-9-THC-D9 11-nor-9-carboxy-Δ-9-THC-D9, CBDV Cannabidivarin, CBG Cannabigerol, CBD Cannabidiol, THCV Tetrahydrocannabivarin, CBN Cannabinol, Δ-9-THC Δ-9-tetrahydrocannabinol, Δ-8-THC Δ-8-tetrahydrocannabinol, CBL Cannabicyclol, CBC Cannabichromene, CBDA Cannabidiolic acid, CBGA Cannabigerolic acid, and THCA Tetrahydrocannabinolic acid) using Intellistart™ feature of MassLynx® or by manual optimization, as necessary. (V voltage, m/z mass-to-charge ratio, IS internal standard)

ISSN: 2522-5782