Table 2 Key properties of GC-FID method for analysing cannabinoids with a capillary column in four different studies

DB5 capillary column

THCA, CBGA, CBCA, THC, CBG, CBC

Initial 60 °C, 3 °C/min, up to 240 °C

Hold for 5 min

Nitrogen

(Romano and Hazekamp 2013)

Silica capillary column coated with DB1

Δ⁹-THC, CBD, CBN, CBG, THCA, CBGA, CBDA

Initial 100 °C, 108 °C/min, up to 280 °C

Hold for 30 min

Nitrogen

(Hazekamp et al. 2009)

DB5 5% diphenyl/95% dimethylpolysiloxane capillary column

THC-THCA

Initial 200 °C, 10 °C/min, up to 300 °C

Hold for 2 min

Helium

(Casiraghi et al. 2018)

Cross-linked poly-5% diphenyl-95% dimethyl polysiloxane capillary column

CBDA-CBD

Initial 45 °C, 2 °C/min, up to 100 °C then 5 °C/min, up to 250 °C.

Hold for 5 min

Helium

(Pellati et al. 2018)