Fig. 3
Effects of cannabinoids on apoptosis. T24 cells were treated for 24 h with either the methanol vehicle, Δ9-tetrahydrocannabinol, cannabidiol, cannabivarin, or cannabichromene. A Histogram showing the % of annexin V-labelled cells and % cells stained for propidium iodide. Cells were counted from three random fields of view on a fluorescence microscope. *p < 0.05, **p < 0.01, n = 3. B Western blotting analysis was performed using an anti-caspase-3 antibody, and β-tubulin was included as a loading control, where a representative blot of n = 3 experiments is shown. Cleaved caspase 3 is indicative of activation of this cascade. C Quantification of the cleaved caspase 3 relative to tubulin from Western blotting analyses. Results represent the means ± SEM of 3 experiments