Fig. 4

Representative chromatograms of A raw flower, B crude CO₂ extract, C crude ethanol extract, D CBD distillate, E mother liquor, and F distillation bottoms. Each matrix sample was spiked with carbaryl, boscalid, and spinosyn A for reference. The ratio of CBDA to CBD occurs in greatest proportion in the raw flower sample, because the biomass is decarboxylated with heat prior to crude extraction. CBD/CBDA was observed in greatest relative abundance for each of the matrices except the distillation bottoms, where CBG occurs in greatest abundance