Fig. 1
From: High-throughput methods to identify male Cannabis sativa using various genotyping methods
Male plant sex marker analysis by qPCR and sequencing. A Box plot analysis of 2nd derivative cycle quotient values for MADC2 and tissue control for 8200 samples from SexID qPCR results. B Genetic alignment of Steep Hill TaqMan probe for male identification from targeted sequencing of 45 males. C Representative amplification plots for Steep Hill sex determination assay (top: MADC2 amplification plot) (bottom: tissue control amplification plot). D Genetic alignment of canonical MADC2 391-bp cloned fragment from Mandolino aligned to queried IDs in PacBio sequence from Pineapple Banana Bubba Kush