Fig. 7

Influence of cannabis fractions on dopamine as mechanism to regulate the avoidance behavior towards 1-octanol. Effects of nematode treatment with the 100 μg/mL of the polar fractions (PFs) of the cannabidiol (CBD)-rich cannabis varieties MGC 1013, MGC 1074, and MGC 1122 on the avoidance of 1-octanol in the presence (green bars) or absence (brown bars) of added dopamine. Nematode treatment with the 100 μg/mL PF of MGC 1013 as well as treatment with a non-toxic dose of 5 μg/mL of the non-polar fraction (NPF) of MGC 1122 alleviates the delayed response to 1-octanol induced by the treatment, suggesting that treatment leads to reduced levels of dopamine and thereby to delayed responsiveness towards aversive 1-octanol. MQ indicates the Milli-Q water control group, DMSO (dimethyl sulfoxide) the vehicle control. Mann-Whitney U tests were used to compare each condition without added dopamine to the same condition with added dopamine. *** = False Discovery Rate (FDR)-corrected p < .001; * = FDR-corrected p < .05