Fig. 6
Effect of cannabis fractions on adverse memory. The effect of polar fractions (PFs; 100 μg/mL) and a non-toxic concentration of the non-polar fractions (decarboxylated (NPFdec; 5 μg/mL) and non-decarboxylated (NPF; 5 μg/mL) of the cannabidiol (CBD)-rich cannabis variety MGC 1013, MGC 1074 and MGC 1122 was tested on adverse memory with conditioning in the presence or absence of butanone. Brown bars indicate the naïve population, green bars the adversely butanone conditioned treatment groups. The PF of MGC 1013 as well as the NPF of MGC 1074 and MGC 1122 reduce the avoidance behavior of nematodes towards butanone (as can be seen by a higher chemotaxis level in conditioned group). MQ indicates the Milli-Q water control group, DMSO (dimethyl sufoxide) the vehicle control. t tests were used to compare the vehicle control conditions (DMSO) to the experimental conditions for each learning condition. * = false discovery rate (FDR)-corrected p < .05