Fig. 4
Effect of polar fractions (PFs) from different varieties on C. elegans motility. The number of full body bends (oscillations) per minute is used as measure for motility after treatment with different varieties from L1 to young adult stage. A effect on motility after exposure of C. elegans to 100 μg/mL PFs of all varieties used in this study: MGC 1003, MGC 1009, and MGC 1101 induce reduced motility in C. elegans after treatment, MGC 1013 increased motility; B effect on motility after exposure of C. elegans to pure cannabinoids containing 0.0143 μg/mL cannabidiol (CBD) (similar to the concentration in the polar fraction (PF) of MGC 1101), pure cannabinoids containing 0.0125 μg/mL CBD (similar to the concentration in the PF of MGC 1074), pure cannabinoids contain 0.0262 μg/mL Δ9-tetrahydrocannabinol acid (THCA) and 0.0133 μg/mL Δ9-tetrahydrocannabinol (THC) (similar to the concentration in the PF of MGC 1101), and pure cannabinoids contain 0.0278 μg/mL THCA & 0.0140 μg/mL THC (similar to the concentration in the PF of MGC 1106). Mann-Whitney U tests were used to compare the vehicle control conditions Dimethyl sulfoxide (DMSO) to the experimental conditions. Milli-Q (MQ) is the water control. Green bars denote motility of C. elegans exposed to PFs of different cannabis varieties; brown bars denote vehicles; ** = false discovery rate (FDR)-corrected p < .01; * = FDR-corrected p < .05