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Table 3 Effect of THC or CBD treatment on liver steatosis in mice fed HFCD

From: THC and CBD affect metabolic syndrome parameters including microbiome in mice fed high fat-cholesterol diet

Group number

ND

HF-C

HF-C CBD

HF-C THC

Treatment

Fed 6 weeks of normal diet

Fed 6 weeks of high-fat lard diet with 1% cholesterol and 0.5% cholic acid

Fed 6 weeks of high-fat lard diet with 1% cholesterol and 0.5% cholic acid and CBD

Fed 6 weeks of high-fat lard diet with 1% cholesterol and 0.5% cholic acid and THC

Animal number

1

2

3

4

5

1

2

3

4

5

1

2

3

4

5

8

1

2

3

4

5

Organ

Liver

Liver

Liver

Liver

Hepatocytic vacuolation, consistent with fatty change — centrilobular

0

0

0

0

0

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

Inflammatory cell infiltration, multifocal

0

0

0

1

1

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

Hepatocellular necrosis, focal

0

0

0

0

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

Bile duct hyperplasia

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

2

0

0

0

0

0

  1. Male C57BL/6J mice aged 7–8 weeks were fed either normal diet (ND), high fat + 1%(w/w) cholesterol + 0.5%(w/w) cholate diet (HF-C), HFCD containing CBD (2.39 mg/kg) (HF-C CBD), HFCD containing THC (2.5 mg/kg) (HF-C THC). At the conclusion of the experiment, livers were collected for analysis. Steatosis grade was scored by the study pathologist, using semiquantitative grading of four grades (0–3), taking into consideration the severity of the changes (0, no lesion; 1, minimal change; 2, mild change; 3, moderate change)