Fig. 7

Effect of THC or CBD treatment on inflammatory gene expression in liver of mice fed HFCD. Male C57BL/6J mice aged 7–8 weeks were fed either normal diet (ND, n = 8), high fat + 1%(w/w) cholesterol + 0.5%(w/w) cholate diet (HF-C, n = 8), HFCD containing CBD (2.39 mg/kg) (HF-C CBD, n = 8), HFCD containing THC (2.5 mg/kg) (HF-C THC, n = 8). At the conclusion of the experiment, RNA was isolated from harvested livers and gene levels quantified using qPCR. Values are expressed as relative expression compared to ND (mean ± SEM). Values marked with different letters are significantly different (p < 0.05) in the Tukey–Kramer test. iNOS, inducible nitric oxide synthase; TNFa, tumor necrosis factor alpha