Table 8 Genotypes for polymorphisms in the rpl32-trnL region (112,153–114,100 bp). Discrepancies between the genotypes obtained by MPS and CE are indicated in parentheses
From: Massively parallel sequencing of Cannabis sativa chloroplast hotspots for forensic typing
Sample name | hSTRa (T) | hSTRa (A) | ndhF-rpl32 INDEL (Variable) | rpl32-trnL hSTR1 (A) | SNPa (A/T) | rpl32-trnL hSTR2 (A) | hSTRa (A) | rpl32-trnL SNP (A/C) | rpl32-trnL hSTR3 (Variable) | rpl32-trnL INDEL (TAAAAA) | SNPa (A/G) |
|---|---|---|---|---|---|---|---|---|---|---|---|
Start location (bp) | 112,294 | 112,551 | 112,562 | 112,830 | 112,900 | 112,961 | 113,017 | 113,044 | 113,149 | 113,246 | 113,459 |
Yoruba Nigeria (NC_027223.1) | 8 | 12 | Absent | 12 | A | 11 | 10 | A | 8 (7T+A) | Present | A |
H2-4 | 8 | 12 | Absent | 12 | A | 11 | 10 | A | 9( 8T+A) | Absent | A |
H3-3 | 8 | 12 | Absent | 12 | A | 11 | 10 | A | 8 (7T+A) | Absent | A |
H5-4 | 8 | 13 | GAATTG+10A | 11 | A | 11 | 10 | C | 6 (6A) | Present | A |
NT H5-1 | 8 | 11 | Absent | 12 | A | 12 | 10 | A | 6 (2T+4A) | Present | A |
NT H5-2 | 8 | 13 | GAATTG+11A | 11 | A | 11 | 10 | C | 6 (6A) | Present | A |
NT H5-4 | 8 | 11 | GAATTG+10A | 11 | T | 11 (CE:12) | 11 | A | 11 (7T+4A) | Present | A |
H8-1 | 8 | 13 | GAATTG+12A | 11 | A | 11 | 10 | C | 6 (6A) | Present | A |
10-A1 | 8 | 13 | GAATTG+11A | 11 | A | 11 | 10 | C | 6 (T+5A) | Present | A |
12-A7 | 8 | 13 | GAATTG+12A | 11 | A | 11 | 10 | C | 6 (6A) | Present | A |
16-B1 | 7 | 11 | GAATTG+10A | 11 | T | 11 (CE:12) | 11 | A | 14 (6T+8A) | Present | G |
21-A16 | 8 | 13 | GAATTG+11A | 10 | A | 11 | 10 | C | 6 (6A) | Present | A |
35 | 7 | 10 | GAATTG+10A | 11 | T | 11 (CE:12) | 11 | A | 15 (11T+4A) | Present | G |
41 | 7 | 10 | GAATTG+10A | 11 | T | 11 (CE:12) | 11 | A | 14 (6T+8A) | Present | G |
MedMJ10 | 8 | 13 | GAATTG+11A | 11 | A | 11 | 10 | C | 6 (6A) | Present | A |