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Table 4 Genotypes for polymorphisms in the trnS-trnG region (8300–9967 bp). Discrepancies between the genotypes obtained by MPS and CE are indicated in parentheses

From: Massively parallel sequencing of Cannabis sativa chloroplast hotspots for forensic typing

Sample name

psbI-trnS hSTR (T)

hSTRa (A)

trnS-trnG SNP1 (T/C)

trnS-trnG SNP2 (A/T)

hSTRa (T)

INDELa (CAATAT)

SNPa (A/T)

trnS-trnG hSTR1 (Variable)

trnS-trnG hSTR2 (T)

hSTRa (A)

trnS-trnG SNP3 (T/A)

SNPa (A/C)

Start location (bp)

8359

8585

8595

8684

8685

8731

8877

9018

9104

9149

9360

9463

Yoruba Nigeria (NC_027223.1)

7

10

T

A

9

Absent

A

15

11

12

A

A

H2-4

7

10

T

A

9

Absent

A

15

11

12

A

A

H3-3

7

10

T

A

9

Absent

A

15

11

12

A

A

H5-4

8

10

C

T

9

Absent

A

15

11

12

T

A

NT H5-1

7

10

T

A

9

Absent

A

15

11

12

T

A

NT H5-2

8

10

C

T

9

Absent

A

15

11

12

T

A

NT H5-4

8

12

T

A

8

Absent

A

16

12 (CE: 11)

11

T

C

H8-1

8

10

C

T

9

Absent

A

15

11

12

T

A

10-A1

8

10

C

T

9

Absent

A

15

11

12

T

A

12-A7

8

10

C

T

9

Absent

A

15

11

12

T

A

16-B1

8

10

T

A

8

Absent

T

16

12 (CE: 11)

11

T

C

21-A16

8

10

C

T

9

Absent

A

15

11

12

T

A

35

8

10

T

A

8

Present

A

16

12 (CE: 11)

11

T

C

41

8

10

T

A

8

Absent

T

16

12 (CE: 11)

11

T

C

MedMJ10

8

10

C

T

9

Absent

A

15

11

12

T

A

  1. Italics indicate polymorphisms not analyzed previously by CE
  2. a Indicates new polymorphisms discovered in this study