Fig. 3
CBGA methyl ester and olivetolic acid do not inhibit GPR55 or T-type calcium channels. A HEK293 cells expressing human GPR55 were used to screen compounds for antagonist activity. ERK phosphorylation (pERK) following treatment with EC80 concentrations of the endogenous ligand LPI was determined by Alphascreen Phospho-ERK1/2 assay and used as a measure of GPR55 activation. Compounds were screened at a concentration of 10 μM (CID, CID16020046). Data are expressed as a mean ± SEM, with n = 5 per group (****p < 0.0001, one-way ANOVA followed by Dunnett’s post hoc). HEK293 cells expressing human B Cav3.1, C Cav3.2, or D Cav3.3 were used to screen compounds for antagonist activity. Changes in intracellular calcium concentrations were used as a measure of calcium channel activity. Compounds were screened at a concentration of 10 μM (NNC, NNC 55-0396). Data are expressed as a mean ± SEM, with n = 5 per group (****p < 0.0001, one-way ANOVA followed by Dunnett’s post hoc)