| Mechanical wound (5-day) | Herbivore (5-day) | Excess heat (7-day) | Drought (7-day) |
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Control (μg/g) | Treatment (μg/g) | Control (μg/g) | Treatment (μg/g) | Control (μg/g) | Treatment (μg/g) | Control (μg/g) | Treatment (μg/g) |
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CBGA | − 130 ± 663 | 172 ± 424 | 308 ± 168 | − 24 ± 47** | 206 ± 147 | − 182 ± 133*** | 265 ± 103 | 34 ± 188 |
CBG | − 2 ± 28 | 49 ± 51 | 69 ± 85 | − 52 ± 43* | 21 ± 29 | − 112 ± 105* | 336 ± 49 | 622 ± 110*** |
CBDA | 611 ± 3216 | 7223 ± 4113 | 671 ± 2108 | − 1367 ± 1053 | − 1948 ± 3065 | − 3081 ± 1442 | 4131 ± 3065 | 826 ± 4417 |
CBD | − 174 ± 354 | 249 ± 162 | 755 ± 397 | 194 ± 121* | − 55 ± 174 | − 338±471 | 1182 ± 60 | 297 ± 267**** |
THCA | 593 ± 3596 | 7112 ± 3671 | 200 ± 2825 | − 806 ± 911 | − 1917 ± 2129 | − 2829±718 | 3201 ± 4801 | 3849 ± 3851 |
THC | − 596 ± 734 | 232 ± 421 | 1771 ± 1034 | 687 ± 301 | − 21 ± 398 | − 1137 ± 1391 | 3927 ± 242 | 580 ± 555**** |
- Floral cannabinoid production differences in the 2 weeks old immature buds in response to mechanical wound, herbivore, excess heat, and drought. Plants were harvested for the cannabinoid analysis 14 days after the transition to the short-day. For statistical analyses, three floral tissues from control- and stress-treated plants were quantified for cannabinoid production. The differences in the cannabinoid level were analyzed by one-way ANOVA, followed by Tukey’s multiple comparisons test. Significantly changed cannabinoid in the stress-treated immature buds are depicted as bold with asterisk in the table (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Note: concentration values reported are the difference between the concentration on day 1 and the concentration on day 6 or 8. A positive value indicates that an increase in that cannabinoid was observed in that 6–8 day period, whereas, a negative value indicates that cannabinoid decreased concentration