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Fig. 1 | Journal of Cannabis Research

Fig. 1

From: Potential impacts of soil microbiota manipulation on secondary metabolites production in cannabis

Fig. 1

This illustration shows the technical workflow for the development of microbial consortia for Cannabis (hemp and marijuana). The source is a microbial suspension from a natural environment rich in microbes. A root colonization study at 4 weeks allows assessment of whether microbes have been established. Next-generation sequencing and the DADA2 pipeline in R can determine community composition, and core microbes can be isolated from cannabis roots and rhizospheric soils. Network analysis will provide insight into the core microbial taxa and hub microbes. Later, the core microbes can be cultured in different microbial growth mediums and microbial synergism can be evaluated. Then, different microbial consortia can be considered for efficacy assessment, and functionally feasible consortia considered for utilization in the cultivation of marijuana and hemp

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