Fig. 3
The number of NeuN positive neurons in the hypothalamus in response to HU-580. Systemic injections of the highest dose of HU-580 (100μg/Kg) increased the number of NeuN positive immunoreactive neurons in the hypothalamus (Panel A; F(2, 12)= 11.334; P< 0.001). The Scheffé´s post hoc test displayed significant differences between the experimental groups for NeuN expression in the hypothalamus (Control vs. HU-580 (0.1μg/Kg), P= 0.2; Control vs. HU-580 (100μg/Kg), P< 0.001; HU-580 (0.1μg/Kg) vs. HU-580 (100μg/Kg), P< 0.04). The Pearson’s correlation coefficient analysis among the doses of HU-580 (0.1 or 100μg/Kg; i.p.) and the NeuN expression showed a significant and positive relationship between these experimental variables (r= 0.5, P< 0.0008; Panel B) whereas the linear regression analysis indicated that HU-580 (0.1 or 100μg/Kg; i.p.) produced a significantly increase in quantitative NeuN neuronal expression in the hypothalamus (R2= 0.5, P< 0.001)