Table 3 Intra-day accuracy and precision for cannabinoids of the assay method. The results verify the repeatability of the assay method as required by AOAC SMRP 2019.003 (Standard Method Performance Requirements (SMPRs) for Quantification of cannabinoids in plant materials of hemp (Low THC Varieties Cannabis sp.) 2019)

Concentration (ng/mL)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% bias)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% bias)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% bias)

10

10.3 ± 0.5

4.6

2.7

10.3 ± 0.5

4.9

3.2

10.5 ± 0.3

3.3

4.8

75

72.3 ± 0.9

1.3

−3.7

72.3 ± 1.4

1.9

−3.6

74.2 ± 2.0

2.7

−1.1

750

768.6 ± 18.7

2.4

2.5

755.6 ± 17.6

2.3

0.7

739.6 ± 20.5

2.8

−1.4

1750

1856.8 ± 31.8

1.7

6.1

1842.8 ± 30.2

1.6

5.3

1760.9 ± 33.8

1.9

0.6

Concentration (ng/mL)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

10

9.2 ± 0.2

2.2

−8.4

11.0 ± 0.4

3.4

10.5

10.5 ± 0.5

4.9

5.1

75

73.2 ± 1.4

1.9

−2.3

69.0 ± 2.2

3.2

−8.1

72.1 ± 0.6

0.9

−3.9

750

767.8 ± 18.6

2.4

2.4

760.2 ± 17.2

2.3

1.4

732.7 ± 15.9

2.2

−2.3

1750

1826.8 ± 26.8

1.5

4.4

1851.1 ± 29.3

1.6

5.8

1754.8 ± 48.6

2.8

0.3

Concentration (ng/mL)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

10

10.3 ± 0.4

3.5

2.6

10.1 ± 0.5

4.6

1.3

10.6 ± 0.4

3.6

5.6

75

76.4 ± 1.4

1.8

1.9

79.1 ± 2.2

2.8

5.4

79.2 ± 1.7

2.1

5.6

750

786.9 ± 16.8

2.1

4.9

757.0 ± 18.1

2.4

0.9

792.9 ± 16.4

2.1

5.7

1750

1833.8 ± 25.0

1.4

4.8

1732.9 ± 13.7

0.8

−1.0

1784.6 ± 23.9

1.3

2.0

Concentration (ng/mL)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

Measured Concentration (mean ± SD)

Precision (%RSD)

Accuracy (% Bias)

10

9.8 ± 0.3

2.9

−1.5

10.3 ± 0.5

4.0

3.4

9.9 ± 0.4

5.5

−0.9

75

79.7 ± 1.7

1.9

6.3

77.6 ± 1.6

2.1

3.5

79.9 ± 1.8

2.3

6.6

750

753.8 ± 16.4

1.3

0.5

710.8 ± 15.5

2.2

−5.2

771.8 ± 14.8

1.9

2.9

1750

1760.7 ± 23.9

0.8

0.6

1625.1 ± 24.0

1.5

−7.1

1763.4 ± 25.6

1.5

0.8