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Table 3 Intra-day accuracy and precision for cannabinoids of the assay method. The results verify the repeatability of the assay method as required by AOAC SMRP 2019.003 (Standard Method Performance Requirements (SMPRs) for Quantification of cannabinoids in plant materials of hemp (Low THC Varieties Cannabis sp.) 2019)

From: Regulatory sampling of industrial hemp plant samples (Cannabis sativa L.) using UPLC-MS/MS method for detection and quantification of twelve cannabinoids

Concentration (ng/mL) Cannabinoid
CBC CBD CBDA
Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% bias) Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% bias) Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% bias)
10 10.3 ± 0.5 4.6 2.7 10.3 ± 0.5 4.9 3.2 10.5 ± 0.3 3.3 4.8
75 72.3 ± 0.9 1.3 −3.7 72.3 ± 1.4 1.9 −3.6 74.2 ± 2.0 2.7 −1.1
750 768.6 ± 18.7 2.4 2.5 755.6 ± 17.6 2.3 0.7 739.6 ± 20.5 2.8 −1.4
1750 1856.8 ± 31.8 1.7 6.1 1842.8 ± 30.2 1.6 5.3 1760.9 ± 33.8 1.9 0.6
Concentration (ng/mL) Cannabinoid
CBDV CBG CBGA
Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias) Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias) Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias)
10 9.2 ± 0.2 2.2 −8.4 11.0 ± 0.4 3.4 10.5 10.5 ± 0.5 4.9 5.1
75 73.2 ± 1.4 1.9 −2.3 69.0 ± 2.2 3.2 −8.1 72.1 ± 0.6 0.9 −3.9
750 767.8 ± 18.6 2.4 2.4 760.2 ± 17.2 2.3 1.4 732.7 ± 15.9 2.2 −2.3
1750 1826.8 ± 26.8 1.5 4.4 1851.1 ± 29.3 1.6 5.8 1754.8 ± 48.6 2.8 0.3
Concentration (ng/mL) Cannabinoid
CBL CBN Δ-8-THC
Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias) Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias) Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias)
10 10.3 ± 0.4 3.5 2.6 10.1 ± 0.5 4.6 1.3 10.6 ± 0.4 3.6 5.6
75 76.4 ± 1.4 1.8 1.9 79.1 ± 2.2 2.8 5.4 79.2 ± 1.7 2.1 5.6
750 786.9 ± 16.8 2.1 4.9 757.0 ± 18.1 2.4 0.9 792.9 ± 16.4 2.1 5.7
1750 1833.8 ± 25.0 1.4 4.8 1732.9 ± 13.7 0.8 −1.0 1784.6 ± 23.9 1.3 2.0
Concentration (ng/mL) Cannabinoid
Δ-9-THC THCA THCV
Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias) Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias) Measured Concentration (mean ± SD) Precision (%RSD) Accuracy (% Bias)
10 9.8 ± 0.3 2.9 −1.5 10.3 ± 0.5 4.0 3.4 9.9 ± 0.4 5.5 −0.9
75 79.7 ± 1.7 1.9 6.3 77.6 ± 1.6 2.1 3.5 79.9 ± 1.8 2.3 6.6
750 753.8 ± 16.4 1.3 0.5 710.8 ± 15.5 2.2 −5.2 771.8 ± 14.8 1.9 2.9
1750 1760.7 ± 23.9 0.8 0.6 1625.1 ± 24.0 1.5 −7.1 1763.4 ± 25.6 1.5 0.8
  1. Precision was measured as the percent relative standard deviation which was calculated by multiplying the standard deviation by 100 then dividing this value by the mean. Accuracy was measured as percent bias which was calculated by subtracting the observed mean from the nominal concentration then dividing this value by the nominal concentration prior to multiplying by 100 to get the percent bias
  2. SD Standard deviation, %RSD Percent relative standard deviation, CBDV Cannabidivarin, CBG Cannabigerol, CBD Cannabidiol, THCV Tetrahydrocannabivarin, CBN Cannabinol, Δ-9-THC Δ-9-tetrahydrocannabinol, Δ-8-THC Δ-8-tetrahydrocannabinol, CBL Cannabicyclol, CBC Cannabichromene, CBDA Cannabidiolic acid, CBGA Cannabigerolic acid, and THCA Tetrahydrocannabinolic acid