Fig. 5

Conjugates dienes from LDL oxidation. a LDL (0.1 mg/mL) was exposed to CuSO₄ (50 μM), and the formation of conjugated dienes was followed at 234 nm in the absence (dashed line) and the presence of cannabis extracts (continuous lines). Number over the curves represent the concentration of extract in μg/mL. Representative curves obtained in the presence of extracts from SB3 (1.25–5 μg/mL) are shown. The dotted line represents control LDL assayed in the same condition but without the addition of cupper ions. b Linear regression of latency ratios and the amount of SB3. The slope of these graphs (antioxidant capacity (AC/μg)) are summarized in Table 3. c Correlation between the AC/μg and the concentration of THC (R² = 0.43). and THC-A (R² = 0.49) in each extract. d Representative plot of percentage of protection, determined as described in Materials and Methods, against the concentration of SB3. Analogous plots obtained for each extract were used to obtain the values of IC₅₀ shown in Table 3