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Table 3 Key properties of common HPLC methods for analysing cannabinoids in eight different studies

From: Methods for quantification of cannabinoids: a narrative review

Column properties Mobile phase Cannabinoids analysed Flow rate mL/min Injection volume Range LOD LOQ References
C18 column Methanol and water, acidified with formic acid THCA, CBGA, CBCA, THC, CBG, CBC 0.5 2 μL 200–400 nm N/A N/A (Romano and Hazekamp 2013)
C18 with a C18 guard column Methanol and water, acidified with formic acid 16 major cannabinoids 0.3 Not stated 280–650 nm N/A N/A (Hazekamp et al. 2005)
C18 with a C18 guard column Methanol and water, acidified with formic acid Δ9-THC, CBD, CBN, CBG, THCA, CBGA, CBDA 1.5 Not stated 200–400 nm N/A N/A (Hazekamp et al. 2009)
C18 column 0.1% formic acid in both (A) H2O and (B) ACN CBDA, CBGA, CBD, CBG 0.4 3 μL 2.5–200(μg/mL) 0.5–0.8 (μg/mL) 1.8–2.5(μg/mL) (Brighenti et al. 2017)
RP18 end-capped column with a guard column 15% of acetic acid in water and methanol CBG, CBD, CBDA, CBN, CBGA, THC, CBC, THCA 1.5 50 μL 220–280 nm N/A N/A (Namdar et al. 2018)
C18 column 0.1% formic acid in both (A) H2O and (B) ACN CBDA-CBD 0.4 3 μL >  0.999 0.4–0.1 μg/mL 1.3–0.3 μg/mL (Pellati et al. 2018)
Silica column, with guard column Hexane/2-propanol/dimethyl propane (1000/5/1, v/v/v) N/A 1.0 20 mL 200–440 nm N/A N/A (Oomah et al. 2002)
C18 end capped column with guard column 15% of acetic acid in water and methanol CBG, CBD, THC, CBC, CBN 1.5 50 μL 220–280 nm N/A N/A (Namdar et al. 2019)