Fig. 4

The effects of cannabis cultivar extracts were examined in the standard hot plate assay 5–10 min post-dose. Mice were intravenously dosed with control (vehicle) or CTL-H01-H3 (0.1, 0.3, 1 and 3 mg/kg) (n = 10 per group), CTL-H01-H2 (0.1, 0.3, 1, 3 and 6 mg/kg) (n = 6 per group), CTL-P01-H1 (0.1, 0.3, 1, 3 and 6 mg/kg) (n = 6 per group with 1 animal excluded from 6 mg/kg), CTL-G01-H8 (0.03, 0.1, 0.3, 1 and 3 mg/kg) (n = 6 per group), CTL-G03-H2 (0.03, 0.1, 0.3, 1 and 3 mg/kg) (n = 6 per group) or CTL-X02-H1 (0.1, 0.3, 1, and 2 mg/kg) (n = 10 per group) cannabis cultivar extract. The curves have been summarized on the same set of axes to facilitate visual comparisons. Data are presented as the mean ± SEM. Data were fit using a 3 parameter log (agonist) vs. response non-linear regression model. Dosing was based on the Δ⁹-THC content of the cannabis cultivar extract, as analyzed by HPLC