Fig. 2

The effects of Δ⁹-THC and morphine in each behavioral assay. Mice were intravenously dosed with control (vehicle) (n = 7), 0.3 (n = 7, 1 animal excluded), 1 (n = 7), 3 mg/kg (n = 7) Δ⁹-THC (panel a) or control (vehicle) (n = 7), 0.3 (n = 7), 1 (n = 7), 3 (n = 7), or 10 mg/kg (n = 7) morphine (panel c) and immobility time in the tail suspension assay was evaluated 20 min post dose. The effects of Δ⁹-THC (n = 7 per group) (panel b) and morphine (n = 7 per group) (panel d) were evaluated 5–10 min post-dose in the standard hot plate assay. Note that the maximal duration is denoted as 120 s in this assay by the dotted lines (panels b and d). Data is presented as the mean ± SEM. Data were fit using a 3 parameter log (agonist) vs. response non-linear regression model