Fig. 1

A time-effect profile of pure (≥98%) Δ⁹-THC on immobility time in the tail suspension test (panel a). Mice were intravenously dosed with control (vehicle) or 0.3, 1, 3, and 10 mg/kg pure Δ⁹-THC (n = 5 per group, 1 animal excluded from 10 mg/kg) and immobility time evaluated for 6 min at various time points at 1, 20, 60, and 120 min. Panel b shows the time-effect curve for Δ⁹-THC in the standard hot plate assay. Mice were administered i.v. with vehicle, 0.3, 1, 3, and 10 mg/kg (n = 5 per group, 1 animal excluded from 10 mg/kg). The minimal time spent immobile at time 0 is denoted by the dotted line in panel a while the maximal latency to licking of the hind paw is 120 s and denoted by the dotted line in panel b. Data is fit using an “eye line of best fit” due to the lack of knowledge regarding the underlying mathematical models. Data are presented as the mean ± SEM for each treatment group. **/††/‡‡ indicates P < 0.01, ***/††† indicates P < 0.001, ****/†††† indicates P < 0.0001. Δ⁹-THC doses were compared to vehicle at the given time point