Table 2 Effect of radiation and sterilization methods on fungal contamination of cannabis plant samples
Treatment | Experiment number | Sample type | Average CFU before treatment [log 10 CFU/g inflorescence] | Radiation dose [kGy] | Plasma exposure time [min] | Average CFU after treatment [log 10 CFU/g inflorescence] |
|---|---|---|---|---|---|---|
Gamma irradiation | 1 | Uninoculated inflorescence | 6.16 ± 0.26 | 7.5 | – | 0 |
1 | Botrytis cinerea-inoculated inflorescence | 8.05 ± 0.12 | 7.5 | – | 1.88 ± 0.96 | |
2 | Uninoculated inflorescence | 6.04 ± 0.08 | 8.37 | – | 0 | |
2 | Botrytis cinerea-inoculated inflorescence | 7.7 ± 0.11 | 8.37 | – | 3.02 ± 0.11 | |
E-beam | 1 | Uninoculated inflorescence | 6.16 ± 0.26 | 10.26 | – | 0 |
1 | Botrytis cinerea-inoculated inflorescence | 8.05 ± 0.12 | 10.26 | – | 0 | |
2 | Uninoculated inflorescence | 6.04 ± 0.08 | 10.26 | – | 0 | |
2 | Botrytis cinerea-inoculated inflorescence | 7.7 ± 0.11 | 10.26 | – | 1.75 ± 0.5 | |
1 | Naturally infected commercial inflorescence | 5 ± 0.25 | 11.99 | – | 0.211 ± 0.1 | |
2 | Naturally infected commercial inflorescence | 2.2 ± 0.47 | 11.99 | – | 0 | |
1 | Naturally infected commercial trimmed leaves | 4.3 ± 0.03 | 11.99 | – | 0.314 ± 0.095 | |
2 | Naturally infected commercial trimmed leaves | 5.27 ± 0.05 | 11.99 | – | 0.6 ± 0.48 | |
Cold plasma | 1 | Uninoculated inflorescence | 3 ± 0.08 | – | 10 | 0 |
2 | Uninoculated inflorescence | 2.81 ± 0.91 | – | 10 | 0.25 ± 0.25 | |
2 | Botrytis cinerea-inoculated inflorescence | 7.82 ± 0.04 | – | 10 | 3.84 ± 0.08 | |
Cold plasma with changing vacuum timea | 1 | Uninoculated inflorescence | 6.6 ± 0.35 | – | 12.5 min + 10 min vacuum | 0.25 ± 0.25 |
1 | Botrytis cinerea-inoculated inflorescence | 6.88 ± 0.11 | – | 10 min + 8 min vacuum | 4.05 ± 0.31 |